Epigenetic research is a fascinating, rapidly evolving field of biology, concerned with modifying gene expression, without modifying the underlying coding sequence. This can include RNA interference, transcription factor regulation, DNA methylation, and my personal favourite, histone modifications.
I use a mixture of molecular, sequencing, and physiological approaches to understanding epigenetic regulation of root system architecture and responses to salt stress in model species Arabidopsis thaliana. This involves growing hundreds of plants in soil, agar plates, or in hydroponics.
These techniques include, but are not limited to:
- Chromatin Immunoprecipitation & Sequencing
- Quantitative PCR
- RNA sequencing
- Gateway and Classical Cloning
- Quantitative Phenotyping
- Confocal microscopy
- EdU staining
- INTACT line generation
- Fluorescent Assisted Cell Sorting (FACS)
- Hormone Assays
Interesting in knowing what these techniques are, and why we use them? Check the blog weekly for guides on each one, its applications, and a basic-how to, including diagrams.
You can find more out about working with plants, their morphology, how they grow and why we study them here.