Hi! If you’re here, you’re probably interested in finding out about my work as a scientist. You can read a brief overview here, in my about section, or you can stick around for the nitty-gritty.

PhD overview:

I’m a PhD Student at the University of Glasgow, fully funded by the highly competitive MVLS DTP program.

I work in Professor Anna Amtmann’s lab, studying epigenetic regulation of tissue specific gene expression, focusing specifically on responses to salt stress in plant roots. I’m currently drafting a paper with a view to publish in Nature Plants, so please keep an eye out!

To do this, I use the following techniques:

Molecular Biology

  • DNA and RNA extraction, cDNA synthesis
  • RT-qPCR and conventional PCR
  • Chromatin Immuno-Precipitation (ChIP)
  • Protein synthesis and isolation in E.coli
  • Western blotting
  • Classical and gateway cloning
  • Agrobacterium mediated plant transformation

Plant growth and culture

  • Sterile plant growth on plates
  • Tissue and callus culture
  • Soil growing
  • Hydroponic culture
  • Liquid seedling culture

Sequencing

  • ChIP-seq
  • RNA-seq
  • Data analysis

Other techniques

  • Protoplast generation
  • Ion quantification with flame spectrophotometry
  • Fluorescence Activated Cell Sorting
  • Confocal imaging and fluorescence quantitation