Hi! If you’re here, you’re probably interested in finding out about my work as a scientist. You can read a brief overview here, in my about section, or you can stick around for the nitty-gritty.

PhD overview:

I’m a PhD Student at the University of Glasgow, fully funded by the highly competitive MVLS DTP program.

I work in Professor Anna Amtmann’s lab, studying epigenetic regulation of tissue specific gene expression, focusing specifically on responses to salt stress in plant roots. I’m currently drafting a paper with a view to publish in Nature Plants, so please keep an eye out!

To do this, I use the following techniques:

Molecular Biology

• DNA and RNA extraction, cDNA synthesis
• RT-qPCR and conventional PCR
• Chromatin Immuno-Precipitation (ChIP)
• Protein synthesis and isolation in E.coli
• Western blotting
• Classical and gateway cloning
• Agrobacterium mediated plant transformation

Plant growth and culture

• Sterile plant growth on plates
• Tissue and callus culture
• Soil growing
• Hydroponic culture
• Liquid seedling culture

Sequencing

• ChIP-seq
• RNA-seq
• Data analysis

Other techniques

• Protoplast generation
• Ion quantification with flame spectrophotometry
• Fluorescence Activated Cell Sorting
• Confocal imaging and fluorescence quantitation